限域纳米孔道电化学传感及其药物分析应用

2013年9月-2018年6月，西南大学，分析化学，博士

2009年9月-2013年6月，河南理工大学，应用化学，学士

2018年7月-至今，南昌大学，药物分析教研室，讲师

一、主要科研成果

围绕生物医学信息分子开展孔道离电传感和机器学习分类研究。主持江西省自然科学基金青年项目1项。以第一作者或通讯作者身份在高水平期刊Environmental Science & Technology, Nano Letters, Analytical Chemistry等发表SCI论文24篇；相关研究成果总被引2000余次。

1、主持科研课题：

[1] 江西省科学技术厅, 青年基金项目, 20224BAB216116, 二维光谱阵列传感试纸同时检测中药中多农药残留研究, 2023-01至2025-12, 10万元, 在研, 主持.

[2] 南昌大学, 青年人才培育创新基金项目, PYQN20230089, 比率荧光探针用于药材中α-葡萄糖苷酶抑制剂高通量快速筛选研究, 2023-07至2026-06, 20万元, 在研,主持.

2、发表代表性论文（第一或通讯作者）：

[1] Li, N.; Pan, Z.; Hao, Z.; Ma, Q.; Yan, Y.; Sui, X.; Li, Y.; Liao, Y.; Mai, X.; Gao, Z.*; Xia, F. Ultrasensitive Nanofluidic Detection of 17β-Estradiol in Natural Water by DNA Circuit-Mediated Hyperbranched DNA Nanowire Dual-Signal Amplification. Environ. Sci. Technol. 2025, 59, 18082-18093.

[2] Li, N.; Pan, Z.; Yan, Y.; Li, Y.; Ma, Q.; Wang, C.; Liu, F.; Mai, X.; Xia, C.; Wei, Q.; Gao, Z.* Spatially Controlled DNA Hydrogel–MXCT Nanofluidic Biosensor for Noninvasive Iontronic Detection of Estradiol across the Menstrual Cycle. Nano Lett., 2026, https://doi.org/10.1021/acs.nanolett.5c06007.

[3] Fan, Y.; Shen, S.*; Su, M.; Yan, H.; Dong, J.; Li, Y.; Li, N.*; Gao, Z.*; Xia, F. Synergistic CRISPR/Cas12a-Nanozyme System for Iontronic Sensing of Site-Specific Septin9 Methylation. Anal. Chem. 2026, https://doi.org/10.1021/acs.analchem.5c05637.

[4] Li, N.; Long, Q.; Sui, X.; Li, Y.; Li, X.; Li, Y.; Zhao, T.; Wang, C.; Liu, F.; Mai, X.; Gao, Z.*; Wei, Q.; Xia, F. DNA nanoribbon-templated copper nanoclusters enable activation of the Nrf2/HO-1 pathway for enhanced combating of oxidative stress. J. Colloid Interface Sci. 2026, 702, 138837.

[5] Liu, A.; Zhang, Y.; Wang, L.; Tu, J.; Fan, Y.; Zhong, Z.; Li, N.*; Gao, Z.* Synergistic hybridization chain reaction and click-chemistry-mediated AuNP amplification for iontronic ultrasensitive 5-mC DNA detection. Sens. Actuators B Chem. 2026, 451, 139431.

[6] Dong, J. X.; Xiao, K.; Wu, X. L.; Zhong, Z. Y.; Yuan, H. Y.; Shen, S. G.; Su, M.; Li, N.*; Gao, Z.*; Xia, F. High quantum yield copper nanoclusters integrated with nitrogen-doped carbon dots for off-on ratiometric fluorescence sensing of S2- and Zn2+. Talanta 2025, 286, 127565.

[7] Wang, Y.-X.; Zhong, Z.-Y.; Ya, J.-P.; Li, X.-Y.; Yan, Y.; Zhao, T.-S.*; Li, N.* Acid phosphatase biosensing via Prussian Blue-Functionalized heterometallic covalent organic framework nanozymes. Spectrochim. Acta, Part A 2025, 343, 126497.

[8] Li, N.; Long, Q. H.; Li, X. Y.; Dong, C.; Zhao, T. S.; Mai, X.; Zhao, Y. S.; Gao, Z.*; Wei, Q.; Xia, F. Concurrent manipulation of competitive mechanisms to construct glutathione-stabilized gold nanocluster-based dual-channel molecular classifier for metal ions detection and information steganography. Talanta 2024, 278, 126526.

[9] Long, Q.-H.; Hong, L.-N.; Han, C.; Li, X.-Y.; Liao, Y.-J.; Yan, X.-L.; Mai, X.*; Li, N.* Eu3+-functionalized covalent organic framework for ratiometric fluorescence detection and adsorption of tetracycline and information steganography. Microchim. Acta 2024, 191, 519.

[10] Li, X.-Y.; Long, Q.-H.; Pan, Z.-J.; Ma, X.-H.; Xia, C.-H.; Mai, X.*; Li, N.* Integrated Eu3+ loaded covalent organic framework with smartphone for ratiometric fluorescence detection of tetracycline. Spectrochim. Acta, Part A 2024, 320, 124610.

[11] Cao, H.-T.; Zhao, T.-S.; Liu, W.; Xu, C.-Y.; Liao, Y.-J.; Yan, X.-L.; Mai, X.*; Li, N.* Smartphone-assisted ratiometric fluorescence sensor for sensitive and portable α-glucosidase activity detection and inhibitor screening. Microchem. J. 2024, 197, 109723.

[12] Li, N.; Zhong, Y.-Q.; Liu, S.-G.; He, Y.-Q.; Fan, Y.-Z.; Hu, J.-H.; Mai, X.* Smartphone assisted colorimetric and fluorescent triple-channel signal sensor for ascorbic acid assay based on oxidase-like CoOOH nanoflakes. Spectrochim. Acta, Part A 2020, 238, 118412.

[13] Li, N.; Hao, X.; Kang, B.-H.; Xu, Z.; Shi, Y.; Li, N.-B.*; Luo, H.-Q.* Enzyme-free fluorescent biosensor for the detection of DNA based on core–shell Fe3O4 polydopamine nanoparticles and hybridization chain reaction amplification. Biosens. Bioelectron. 2016, 77, 525–529.

[14] Li, N.; Liu, S.-G.; Zhu, Y.-D.; Liu, T.; Lin, S.-M.; Shi, Y.; Luo, H.-Q.*; Li, N.-B.* Tuning gold nanoparticles growth via DNA and carbon dots for nucleic acid and protein detection. Sens. Actuators, B 2017, 251, 455–461.

[15] Li, N.; Liu, S.-G.; Fan, Y.-Z.; Ju, Y.-J.; Xiao, N.; Luo, H.-Q.*; Li, N.-B.* Adenosine-derived doped carbon dots: from an insight into effect of N/P co-doping on emission to highly sensitive picric acid sensing. Anal. Chim. Acta 2018, 1013, 63–70.

[16] Li, N.; Liu, S.-G.; Dong, J.-X.; Fan, Y.-Z.; Ju, Y.-J.; Luo, H.-Q.*; Li, N.-B.* Using high-energy phosphate as energy-donor and nucleus growth-inhibitor to prepare carbon dots for hydrogen peroxide related biosensing. Sens. Actuators, B 2018, 262, 780–788.

3、人才培养：

[1] 以第一指导老师身份指导学生钟志英等获得第十届全国大学生生命科学竞赛(科学探究类)国家级二等奖（2025年）

[2] 指导学生卓艳婷获得第八届全国医药院校药学/中药学专业人学生实验技能竞赛二等奖（2024年）

[3] 指导研究生龙庆红、潘周健获研究生国家奖学金